Moffat Lab Publications since 2001

2005

Ihee H, Rajagopal S, Srajer V, Pahl R, Anderson S, Schmidt M, Schotte F, Anfinrud PA, Wulff M, Moffat K. Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds. Proc Natl Acad Sci U S A. 2005. (pdf)

Key J, Moffat K. Crystal structures of deoxy and CO-bound bjFixLH reveal details of ligand recognition and signaling. Biochemistry. 2005 Mar 29;44(12):4627-35. (pdf)

Rajagopal S, Anderson S, Srajer V, Schmidt M, Pahl R, Moffat K. A structural pathway for signaling in the E46Q mutant of photoactive yellow protein. Structure (Camb). 2005 Jan;13(1):55-63. (pdf)

2004

Rajagopal S, Key JM, Purcell EB, Boerema DJ, Moffat K. Purification and initial characterization of a putative blue light-regulated phosphodiesterase from Escherichia coli. Photochem Photobiol. 2004 Nov-Dec;80(3):542-7. (pdf)

Rajagopal S, Kostov KS, Moffat K. Analytical trapping: extraction of time-independent structures from time-dependent crystallographic data. J Struct Biol. 2004 Sep;147(3):211-22. (pdf)

Anderson S, Srajer V, Moffat K. Structural heterogeneity of cryotrapped intermediates in the bacterial blue light photoreceptor, photoactive yellow protein. Photochem Photobiol. 2004 Jul-Aug;80:7-14. (pdf)

Anderson S, Srajer V, Pahl R, Rajagopal S, Schotte F, Anfinrud P, Wulff M, Moffat K.Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein. Structure (Camb). 2004 Jun;12(6):1039-45. (pdf)

Anderson S, Crosson S, Moffat K. Short hydrogen bonds in photoactive yellow protein. Acta Crystallogr D Biol Crystallogr. 2004 Jun;60(Pt 6):1008-16. (pdf)

Rajagopal S, Schmidt M, Anderson S, Ihee H, Moffat K. Analysis of experimental time-resolved crystallographic data by singular value decomposition. Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):860-71. (pdf)

Baxter RH, Ponomarenko N, Srajer V, Pahl R, Moffat K, Norris JR. Time-resolved crystallographic studies of light-induced structural changes in the photosynthetic reaction center. Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):5982-7. (pdf)

Kennis JT, van Stokkum IH, Crosson S, Gauden M, Moffat K, van Grondelle R. The LOV2 domain of phototropin: a reversible photochromic switch. J Am Chem Soc. 2004 Apr 14;126(14):4512-3. (pdf)

Schmidt M, Pahl R, Srajer V, Anderson S, Ren Z, Ihee H, Rajagopal S, Moffat K. Protein kinetics: structures of intermediates and reaction mechanism from time-resolved x-ray data. Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):4799-804. (pdf)

2003

Moffat K, Chait BT. Biophysical methods: doing more with less. Curr Opin Struct Biol. 2003 Oct;13(5):535-7. (pdf)

Kennis JT, Crosson S, Gauden M, van Stokkum IH, Moffat K, van Grondelle R. Primary reactions of the LOV2 domain of phototropin, a plant blue-light photoreceptor. Biochemistry. 2003 Apr 1; 42(12): 3385-92. (pdf)

Schmidt M, Rajagopal S, Ren Z, Moffat K. Application of singular value decomposition to the analysis of time-resolved macromolecular x-ray data. Biophys J. 2003 Mar;84(3):2112-29. (pdf)

Rajagopal S, Moffat K. Crystal structure of a photoactive yellow protein from a sensor histidine kinase: conformational variability and signal transduction. Proc Natl Acad Sci U S A. 2003 Feb 18; 100(4): 1649-54. (pdf)

Moffat K. The frontiers of time-resolved macromolecular crystallography: movies and chirped X-ray pulses. Faraday Discuss. 2003;122:65-77; discussion 79-88.

Crosson S, Rajagopal S, Moffat K. The LOV domain family: photoresponsive signaling modules coupled to diverse output domains. Biochemistry. 2003 Jan 14;42(1):2-10. (pdf)

2002

Teng TY, Moffat K. Radiation damage of protein crystals at cryogenic temperatures between 40 K and 150 K. J Synchrotron Radiat. 2002 Jul 1;9(Pt 4):198-201. 

Crosson S, Moffat K. Photoexcited structure of a plant photoreceptor domain reveals a light-driven molecular switch. Plant Cell. 2002 May;14(5):1067-75. (pdf)

2001

Moffat K. Time-resolved biochemical crystallography: a mechanistic perspective. Chem Rev. 2001 Jun;101(6):1569-81. (pdf)

Srajer V, Ren Z, Teng TY, Schmidt M, Ursby T, Bourgeois D, Pradervand C, Schildkamp W, Wulff M, Moffat K. Protein conformational relaxation and ligand migration in myoglobin: a nanosecond to millisecond molecular movie from time-resolved Laue X-ray diffraction. Biochemistry. 2001 Nov 20;40(46):13802-15. (pdf)

Ren Z, Perman B, Srajer V, Teng TY, Pradervand C, Bourgeois D, Schotte F, Ursby T, Kort R, Wulff M, Moffat K. A molecular movie at 1.8 A resolution displays the photocycle of photoactive yellow protein, a eubacterial blue-light receptor, from nanoseconds to seconds. Biochemistry. 2001 Nov 20;40(46):13788-801. (pdf)

Crosson S, Moffat K. Structure of a flavin-binding plant photoreceptor domain: insights into light-mediated signal transduction. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):2995-3000. (pdf)